Generator

Part:BBa_K2694000:Design

Designed by: Jessica Larocque   Group: iGEM18_NDC-HighRiverAB   (2018-10-10)


EstA generator under control of pLac promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 296
    Illegal BglII site found at 443
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 226
    Illegal NgoMIV site found at 682
    Illegal NgoMIV site found at 820
    Illegal NgoMIV site found at 1360
    Illegal NgoMIV site found at 1729
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This circuit was designed to produce the biobrick BBa_K2694001. The IPTG inducible LacI promoter and a strong ribosome binding site. A double terminator was used at the end of the circuit to prevent continued read-through.

Source

Wilhelm et al. (1999). Synthesized through BioBasic.

References

Wilhelm S., Thomassen J., & Jaeger K.E. (1999). A Novel Lipolytic Enzyme Located in the Outer Membrane of Pseudomonas aeruginosa. Journal of Bacteriology. 6977-86.